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Human PDGFRB Antibody Pair - BSA and Azide free (ab256701)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human, Monkey

Overview

  • Product name

    Human PDGFRB Antibody Pair - BSA and Azide free
    See all PDGFR beta kits

  • Assay type

    ELISA set

  • Range

    156.25 pg/ml - 10000 pg/ml

  • Species reactivity

    Reacts with: Human, Monkey

  • Product overview

    The Antibody Pair can be used to quantify Human PDGFRB. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab252357), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human PDGFRB Capture Antibody (unconjugated) 1 x 100µg
    Human PDGFRB Detector Antibody (unconjugated) 1 x 100µg

  • Research areas

  • Function

    Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.

  • Involvement in disease

    Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
    Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
    Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
    Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
    Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • Beta platelet derived growth factor receptor
    • Beta-type platelet-derived growth factor receptor
    • CD 140B
    • CD140 antigen-like family member B
    • CD140b
    • CD140b antigen
    • IBGC4
    • IMF1
    • JTK12
    • OTTHUMP00000160528
    • PDGF R beta
    • PDGF Receptor beta
    • PDGF-R-beta
    • PDGFR
    • PDGFR 1
    • PDGFR beta
    • PDGFR1
    • PDGFRB
    • PGFRB_HUMAN
    • Platelet derived growth factor receptor 1
    • Platelet derived growth factor receptor beta
    • Platelet derived growth factor receptor beta polypeptide
    see all

  • Database links

    Images

    • Sandwich ELISA - Human PDGFRB Antibody Pair - BSA and Azide free (ab256701)
      Sandwich ELISA - Human PDGFRB Antibody Pair - BSA and Azide free (ab256701)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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