Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human PD-L1 ELISA Kit [28-8] (ab277712)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.75 pg/ml
  • Range: 7.81 pg/ml - 500 pg/ml
  • Sample type: Cell culture supernatant, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human PD-L1 ELISA Kit [28-8]
    See all PD-L1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Supernatant 8 2.7%
    Inter-assay
    Sample n Mean SD CV%
    Supernatant 3 4.3%

  • Sample type

    Cell culture supernatant, Urine, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    3.75 pg/ml

  • Range

    7.81 pg/ml - 500 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93 82% - 100%
    Urine 93 86% - 97%
    Serum 96 94% - 97%
    Cell culture extracts 102 99% - 106%
    Hep Plasma 101 90% - 111%
    EDTA Plasma 90 89% - 92%
    Cit plasma 90 87% - 95%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human PD-L1 ELISA kit [28-8] (ab277712) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PD-L1 protein in human serum, plasma, cell culture supernatant, urine, and cell extract samples. It uses our proprietary SimpleStep ELISA® technology and our recombinant PD-L1 antibody clone [28-8]. Quantitate Human PD-L1 with 3.75 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    PD-L1 (also known as CD274 or B7-H1) is a membrane bound glycoprotein involved in regulation of the immune system. PD-L1 is expressed on a variety of inflammatory-activated cells as well as some carcinomas and in melanoma. PD-L1 binds to PD-1 and CD80, where it can suppress T cell activation and proliferation as well as induce apoptosis. Levels of PD-L1 are increased in the plasma of cancer patients as well as in cerebrospinal fluid of gliomas. PD-L1 can bind PD-1 in order to regulate T cell apoptosis.  

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human PD-L1 [28-8] Capture Antibody 1 x 600µl
    10X Human PD-L1 [28-8] Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human PD-L1 [28-8] Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

  • Tissue specificity

    Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Cell membrane and Endomembrane system.
  • Information by UniProt

  • Alternative names

    • B7 H
    • B7 H1
    • B7 homolog 1
    • B7-H1
    • B7H
    • B7H1
    • CD 274
    • CD274
    • CD274 antigen
    • CD274 molecule
    • MGC142294
    • MGC142296
    • OTTHUMP00000021029
    • PD L1
    • PD-L1
    • PD1L1_HUMAN
    • PDCD1 ligand 1
    • PDCD1L1
    • PDCD1LG1
    • PDL 1
    • PDL1
    • Programmed cell death 1 ligand 1
    • Programmed death ligand 1
    • RGD1566211
    see all

  • Database links

    Images

    • SimpleStep ELISA Protocol Diagram
      SimpleStep ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

    • . Example of human PD-L1 standard curve in Sample Diluent NS. The PD-L1 standard curve was prepared as described in Section 10.
      . Example of human PD-L1 standard curve in Sample Diluent NS. The PD-L1 standard curve was prepared as described in Section 10.

      Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Example of human PD-L1 standard curve in 1X Cell Extraction Buffer PTR. The PD-L1 standard curve was prepared as described in Section 10.
      Example of human PD-L1 standard curve in 1X Cell Extraction Buffer PTR. The PD-L1 standard curve was prepared as described in Section 10.

      Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • . Interpolated concentrations
      . Interpolated concentrations

      Undiluted samples are as follows: serum 95% and PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L1 concentration was determined to be 111.81 pg/mL in serum and 361.74 pg/mL in stimulated PBMC supernatant.

    • Interpolated concentrations of native PD-L1 in human HDLM-2 cell extract based on a 10 µg/mL extract load.
      Interpolated concentrations of native PD-L1 in human HDLM-2 cell extract based on a 10 µg/mL extract load.

      The concentrations of PD-L1 were measured in duplicate and interpolated from the PD-L1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L1 concentration was determined to be 393.85 pg/mL in HDLM-2 extract.

    • Serum from ten individual healthy human male donors was measured in duplicate.
      Serum from ten individual healthy human male donors was measured in duplicate.

      Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L1 concentration was determined to be 99.45 pg/mL with a range of 59.76 – 168.01 pg/mL.

    • Recombinant Antibody Benefits
      Recombinant Antibody Benefits

      To learn more about the advantages of recombinant antibodies see here.

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