Human P-selectin ELISA kit (CD62P) (ab273160)
Key features and details
- Sensitivity: 3.6 ng/ml
- Range: 4.4 ng/ml - 140 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Other biological fluids, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human P-selectin ELISA kit (CD62P)
See all CD62P kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% A 6 90.4ng/ml 5 5.6% B 6 39.6ng/ml 3.1 7.8% Inter-assay Sample n Mean SD CV% A 18 92ng/ml 6.9 7.5% B 18 42.3ng/ml 1.8 4.4% -
Sample type
Cell culture supernatant, Serum, Other biological fluids, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.6 ng/ml -
Range
4.4 ng/ml - 140 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human P-selectin ELISA kit (CD62P) (ab273160) is for the in-vitro qualitative and quantitative determination of CD62P in supernatants, buffered solutions or serum and plasma samples. This assay will recognize both natural and recombinant human CD62P.
A capture antibody highly specific for CD62P has been coated to the wells of the microtiter strip plate. Binding of CD62P in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed.
During the next incubation period the binding of the biotinylated anti-CD62P secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed.
The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing.
A chromogen substrate is added to the wells resulting in the progressive development of a blue colored complex with the conjugate. The color development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced colored complex is directly proportional to the concentration of CD62P present in the samples and standards.
The absorbance of the color complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of CD62P in any sample tested.
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Tested applications
Suitable for: ELISAmore details -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests Biotinylated Antibody CD62P 1 x 400µl Biotinylated Antibody diluent 1 x 7ml CD62P Coated Microwell strips 1 unit CD62P Standard 2 vials HRP Diluent 1 x 23ml Plastic Plate Cover 2 units Standard diluent Buffer 1 x 25ml Stop Reagent 1 x 11ml Streptavidin-HRP 2 x 5µl TMB Substrate 1 x 11ml Wash Buffer 1 x 10ml -
Research areas
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Function
Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. -
Tissue specificity
Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. -
Involvement in disease
Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
Sequence similarities
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 9 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Antigen CD62
- CD62
- CD62 antigen-like family member P
see all -
Database links
- Entrez Gene: 6403 Human
- Omim: 173610 Human
- SwissProt: P16109 Human
- Unigene: 73800 Human
Images
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Example data
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example of Human P-selectin ELISA kit (CD62P) standard curve.