Human OMD ELISA kit (ab277442)
Key features and details
- Sensitivity: 1.4 ng/ml
- Range: 1.229 ng/ml - 300 ng/ml
- Sample type: Cell culture supernatant, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human OMD ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Hep Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.4 ng/ml -
Range
1.229 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 86.79 76% - 101% Plasma 71.72 67% - 77% Cell culture media 89.67 76% - 98% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human OMD ELISA Kit (ab277442) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human OMD in serum (human OMD concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended as anticoagulants for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Human OMD coated on a 96-well plate. Standards and samples are pipetted into the wells and Human OMD present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human OMD antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human OMD bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 150X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Assay Diluent C 1 x 30ml Biotinylated Anti-Human OMD Antibody 2 vials Anti-Human OMD coated Microplate 1 unit Human OMD standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
OMD may be implicated in the biomineralization processes. It has a function in binding of osteoblasts via the alpha(V)beta(3)-integrin. -
Cellular localization
Secreted, extracellular space, extracellular matrix. -
Alternative names
- Keratan sulfate proteoglycan osteomodulin
- KSPG osteomodulin
- OSAD
see all -
Database links
- Entrez Gene: 4958 Human
- SwissProt: Q99983 Human
- Unigene: 94070 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.