Human LECT2 ELISA Kit (ab269554)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.706 ng/ml
- Range: 1.56 ng/ml - 100 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human LECT2 ELISA Kit
See all LECT2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.7% Inter-assay Sample n Mean SD CV% Serum 3 7.4% -
Sample type
Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.706 ng/ml -
Range
1.56 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94 91% - 96% Tissue Extracts 120 120% - 120% Hep Plasma 101 99% - 103% EDTA Plasma 115 113% - 118% Cit plasma 114 111% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Cow -
Product overview
Human LECT2 ELISA Kit (ab269554) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LECT2 protein in hep plasma, serum, cit plasma, tissue extracts, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LECT2 with 0.706 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Leukocyte cell derived chemotaxin 2 (LECT2) is a highly conserved protein that functions with major development in liver regeneration, immune modulation, bone growth, neuronal development, cancer, and several more in pathophysiology. Studies have shown LECT2 to be a positive regulator of chondrocyte proliferation. Moreover, the liver is considered to be the source of LECT2 due to exceptionally high levels of mRNA. However, expression in these cells is extremely low or undetectable suggesting that hepatocytes secrete LECT2 instantly after production.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human LECT2 Capture Antibody 1 x 600µl 10X Human LECT2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human LECT2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Has a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation. -
Tissue specificity
Highly expressed in adult and fetal liver and weakly in testis. Not expressed in bone marrow. -
Sequence similarities
Belongs to the LECT2/MIM-1 family. -
Cellular localization
Cytoplasm. Secreted. - Information by UniProt
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Alternative names
- chm II
- chm2
- Chondromodulin II
see all -
Database links
- Entrez Gene: 3950 Human
- Omim: 602882 Human
- SwissProt: O14960 Human
- Unigene: 512580 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human LECT2 standard curve in Sample Diluent NS.The LECT2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native LECT2 in human serum and plasma samples.The concentrations of LECT2 were measured in duplicates, interpolated from the LECT2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma-citrate 25%, plasma-EDTA 25%, and plasma-heparin 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LECT2 concentration was determined to be 28.4 ng/mL in serum, 22.5 ng/mL in plasma-citrate, 32.5 ng/mL in plasma-EDTA, and 13.0 ng/mL in plasma-heparin.
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Serum from ten individual healthy human female donors were measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LECT2 concentration was determined to be 51 ng/mL with a range of 20 - 125 ng/mL.
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Interpolated concentrations of native LECT2 in human lung extract based on a 1,000 µg/mL extract load.The concentrations of LECT2 were measured in duplicate and interpolated from the LECT2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LECT2 concentration was determined to be 8.8 ng/mL in lung extract.
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Sandwich ELISA - Human LECT2 ELISA Kit (ab269554)To learn more about the advantages of recombinant antibodies see here.
