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Human NFATC3 (NFAT4) knockout HEK293T cell pellet (ab279024)

Overview

  • Product name

    Human NFATC3 (NFAT4) knockout HEK293T cell pellet
    See all NFAT4/NF-ATc3 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3.

  • Passage number

  • Knockout validation

    Sanger Sequencing

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

Target

  • Function

    Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2.

  • Tissue specificity

    Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.

  • Sequence similarities

    Contains 1 RHD (Rel-like) domain.

  • Domain

    Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.

  • Post-translational
    modifications

    Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.

  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt

  • Alternative names

    • C80703
    • cytoplasmic 3
    • D8Ertd281e
    • NF AT4
    • NF ATc3
    • NF-AT4
    • NF-ATc3
    • NFAC3_HUMAN
    • NFAT4
    • NFATc3
    • NFATx
    • Nuclear factor of activated T cells, cytoplasmic 3
    • nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3
    • Nuclear factor of activated T-cells
    • T cell transcription factor NFAT4
    • T-cell transcription factor NFAT4
    see all

Properties

Images

  • Sanger Sequencing - Human NFATC3 (NFAT4) knockout HEK293T cell pellet (ab279024)
    Sanger Sequencing - Human NFATC3 (NFAT4) knockout HEK293T cell pellet (ab279024)
    Homozygous: 1 bp insertion in exon3

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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