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Human MVP knockout HeLa cell pellet (ab279081)

Overview

  • Product name

    Human MVP knockout HeLa cell pellet
    See all MVP kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HeLa

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 6 and 1 bp deletion in exon 6.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.

  • Tissue specificity

    Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.

  • Sequence similarities

    Contains 9 MVP (vault) repeats.

  • Domain

    MVP 3 mediates interaction with PTEN.
    MVP 4 mediates interaction with PARP4.

  • Post-translational
    modifications

    Phosphorylated on Tyr residues after EGF stimulation.
    Dephosphorylated by PTPN11.

  • Cellular localization

    Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
  • Information by UniProt

  • Alternative names

    • LRP
    • Lung resistance related protein
    • Lung resistance-related protein
    • Major vault protein
    • Major vault protein, rat, homolog of
    • MVP
    • MVP_HUMAN
    • testicular secretory protein Li 30
    • VAULT 1
    • VAULT1
    see all

Properties

Images

  • Western blot - Human MVP knockout HeLa cell pellet (ab279081)
    Western blot - Human MVP knockout HeLa cell pellet (ab279081)

    Lane 1: Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate (20 ug)

    Lane 2: MVP knockout HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate (20 ug)

    Lane 3: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate (20 ug)

    Lanes 1-3: Merged signal (red and green). Green - ab273093 observed at 110 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

    ab273093 Anti-MVP antibody [EPR23594-106] was shown to specifically react with MVP in wild-type HeLa cells in Western blot. Significant decrease (8.7 % of intensity compared to the WT band) of signal was observed when MVP knockout cell line ab264817 (knockout cell lysate ab257544) was used.

    ab273093 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Human MVP knockout HeLa cell pellet (ab279081)
    Western blot - Human MVP knockout HeLa cell pellet (ab279081)

    Lane 1: Wild-type HeLa cell lysate (20 µg)

    Lane 2: MVP knockout HeLa cell lysate (20 µg)

    Lane 3: A549 cell lysate (20 µg)

    Lane 4: MOLT-4 cell lysate (20 µg)

    Lanes 1-4: Merged signal (red and green). Green - ab177145 observed at 99 kDa. Red - loading control ab8245 observed at 37 kDa.

    ab177145 Anti-MVP antibody [EPR13226(B)] was shown to specifically react with MVP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264817 (knockout cell lysate ab257544) was used. Wild-type and MVP knockout samples were subjected to SDS-PAGE. ab177145 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Human MVP knockout HeLa cell pellet (ab279081)
    Western blot - Human MVP knockout HeLa cell pellet (ab279081)

    Lane 1: Wild-type HeLa cell lysate (20 µg)

    Lane 2: MVP knockout HeLa cell lysate (20 µg)

    Lane 3: A549 cell lysate (20 µg)

    Lane 4: MOLT-4 cell lysate (20 µg)

    Lanes 1-4: Merged signal (red and green). Green - ab175239 observed at 110 kDa. Red - loading control ab8245 observed at 37 kDa.

    ab175239 Anti-MVP antibody [EPR13227(B)] was shown to specifically react with MVP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264817 (knockout cell lysate ab257544) was used. Wild-type and MVP knockout samples were subjected to SDS-PAGE. ab175239 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human MVP knockout HeLa cell pellet (ab279081)
    Sanger Sequencing - Human MVP knockout HeLa cell pellet (ab279081)

    Allele-1: 17 bp deletion in exon 6

     

  • Sanger Sequencing - Human MVP knockout HeLa cell pellet (ab279081)
    Sanger Sequencing - Human MVP knockout HeLa cell pellet (ab279081)

    Allele-2: 1 bp deletion in exon 6

     

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