Human MTHFD2 knockout HEK293T cell pellet (ab279109)
Overview
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Product name
Human MTHFD2 knockout HEK293T cell pellet
See all MTHFD2 kits -
Product overview
Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.
Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation. -
Parental Cell Line
HEK293T -
Organism
Human -
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1 and 5 bp deletion in exon1. -
Passage number
Knockout validation
Sanger Sequencing, Western Blot (WB)Notes
Pellet size: 5 million cells/vial.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Tested applications
Suitable for: WBmore detailsProperties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human MTHFD2 knockout HEK293T cell pellet 1 vial Human wild-type HEK293T cell pellet 1 vial -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Target
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Sequence similarities
Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family. -
Developmental stage
Expressed only in developing normal tissues. -
Cellular localization
Mitochondrion. - Information by UniProt
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Alternative names
- Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
- Descriptions
- Methenyltetrahydrofolate cyclohydrolase
see all
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human MTHFD2 knockout HEK293T cell pellet 1 vial Human wild-type HEK293T cell pellet 1 vial -
Research areas
-
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human MTHFD2 knockout HEK293T cell pellet (ab279109)
Lane 1:Wild-type HEK293T cell lysate (20 ug)
Lane 2:MTHFD2 knockout HEK293T cell lysate (20 ug)
Lane 3:HepG2 cell lysate (20 ug)
Lane 4:HeLa cell lysate (20 ug)ab56772 was shown to specifically react with MTHFD2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266383 (knockout cell lysate ab258528) was used. Wild-type and MTHFD2 knockout samples were subjected to SDS-PAGE. ab56772 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated at room temperature for 2.5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human MTHFD2 knockout HEK293T cell pellet (ab279109)Allele-1: 5 bp deletion in exon1
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Sanger Sequencing - Human MTHFD2 knockout HEK293T cell pellet (ab279109)Allele-2: 1 bp deletion in exon1
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Sanger Sequencing - Human MTHFD2 knockout HEK293T cell pellet (ab279109)Allele-3: 1 bp insertion in exon1