Human MSH6 knockout HeLa cell pellet (ab278881)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human MSH6 knockout HeLa cell pellet 1 vial Human wild-type HeLa cell pellet 1 vial -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human MSH6 knockout HeLa cell pellet (ab278881)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: MSH6 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab14204 observed at 160 kDa. Red - loading control ab52866 observed at 50 kDa.
ab14204 Anti-MSH6 antibody [44] was shown to specifically react with MSH6 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255410 (knockout cell lysate ab263763) was used. Wild-type and MSH6 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab14204 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human MSH6 knockout HeLa cell pellet (ab278881)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: MSH6 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab92471 observed at 160 kDa. Red - loading control ab130007 observed at 124 kDa.
ab92471 Recombinant Anti-MSH6 antibody [EPR3945] was shown to specifically react with MSH6 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255410 (knockout cell lysate ab263763) was used. Wild-type and MSH6 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab92471 and Anti-Vinculin antibody [VIN-54] were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human MSH6 knockout HeLa cell pellet (ab278881)Homozygous: 1 bp insertion in exon 4