Human MIS RII ELISA Kit (AMHR2) (ab267622)
Key features and details
- Sensitivity: 0.05 ng/ml
- Range: 0.041 ng/ml - 10 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MIS RII ELISA Kit (AMHR2) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
0.05 ng/ml -
Range
0.041 ng/ml - 10 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 93.49 86% - 102% Plasma 75.52 70% - 82% Cell culture media 95.52 85% - 106% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human MIS RII ELISA Kit is designed for the quantitative determination of MIS RII in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human MIS RII coated on a 96-well plate. Standards and samples are pipetted into the wells and MIS RII present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MIS RII antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIS RII bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human 4-1BB Ligand (TNFSF9), Activin RIIB, Aminopeptidase P2 (XPNPEP2), BAMBI (NMA), BOC, Brevican, Carbonic Anhydrase XII, Carboxypeptidase A2, CD300c (LMIR2), CD320 (TCblR, 8D6A), CDNF, CDO, CHST1, CHST4, CILP1, CNTF R alpha, CRIM1, CRTAC1, CXADR, Dopa Decarboxylase (DDC), DPPII (QPP, DPP7), DSPG3, EMR2, FCAR (CD89), FCRL1(FcRH1), FCRL2 (FcRH2), Gas6, GPR56, GPVI, Hepsin, ILT2 (CD85j), Jagged 2, Kirrel3 (NEPH2), KLF4, LAIR1, LAMP, LAMP1 (CD107a), MDGA1, Neurexin 3.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 150X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Human MIS RII coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human MIS RII Detection Antibody 2 vials Human MIS RII Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for anti-Muellerian hormone. -
Involvement in disease
Defects in AMHR2 are the cause of persistent Muellerian duct syndrome type 2 (PMDS2) [MIM:261550]. PMDS2 is a form of male pseudohermaphroditism characterized by a failure of Muellerian duct regression in otherwise normal males. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- AMH type II receptor
- AMHR
- AMHR2
see all -
Database links
- Entrez Gene: 269 Human
- Omim: 600956 Human
- SwissProt: Q16671 Human
- Unigene: 659889 Human
Images
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Example standard curve
These standard curves are for demonstration only. A standard curve must be run with each assay