Human MIP5 ELISA Kit (ab217774)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 27.1 pg/ml
- Range: 89.4 pg/ml - 1500 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Other biological fluids, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MIP5 ELISA Kit
See all Macrophage inflammatory protein 5 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human serum 3 3.2% Inter-assay Sample n Mean SD CV% Human serum 5 7% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Other biological fluids, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
27.1 pg/ml -
Range
89.4 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 104 97% - 110% Serum 92 90% - 95% Cell culture extracts 106 100% - 115% Other biological fluids 117 116% - 117% Tissue Extracts 108 91% - 121% Hep Plasma 108 102% - 112% EDTA Plasma 107 102% - 115% Cit plasma 103 91% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human MIP5 ELISA Kit (ab217774) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIP5 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, other biological fluids, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MIP5 with 27.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
MIP5 is a secreted monomeric chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. MIP5 acts mainly via CC chemokine receptor CCR1. It also binds to CCR3. MIP5 can be cleaved into 3 chains, MIP5(22-92), MIP5(25-92) and MIP5(29-92). These cleavage products are more potent chemo-attractants than MIP5. They are also found in high levels in synovial fluids from rheumatoid patients.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human MIP5 Capture Antibody 1 x 600µl 10X Human MIP5 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human MIP5 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15. -
Tissue specificity
Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-C motif chemokine 15
- C-C motif chemokine ligand 15
- CC chemokine 3
see all -
Database links
- Entrez Gene: 6359 Human
- Omim: 601393 Human
- SwissProt: Q16663 Human
- Unigene: 272493 Human
Images
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Other - Human MIP5 ELISA Kit (ab217774)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human MIP5 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human MIP5 standard curve in 1X Cell Extraction Buffer PTR.The MIP5 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MIP5 in human serum and plasma samples.The concentrations of MIP5 were measured in duplicates, interpolated from the MIP5 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 20%, plasma (citrate) 20%, plasma (heparin) 20% and plasma (EDTA) 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP5 concentration was determined to be 8705 pg/mL in neat serum, 7196 pg/mL in neat plasma (citrate), 6848 pg/mL in neat plasma (heparin), and 7231 pg/mL in neat plasma (EDTA).
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Interpolated concentrations of native MIP5 in HepG2 (4 days) cell culture supernatant and human synovial fluid samples.The concentrations of MIP5 were measured in duplicates, interpolated from the MIP5 standard curves and corrected for sample dilution. Undiluted samples are as follows: HepG2 cell culture supernatant 100% (neat), and synovial fluid 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP5 concentration was determined to be 706.2 pg/mL in neat HepG2 cell culture supernatant and 5,385 pg/mL in neat synovial fluid.
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Interpolated concentrations of native MIP5 in HT-29 cell extract based on a 300 µg/mL extract load and in human colon tissue extract based on a 500 µg/mL extract load.The concentrations of MIP5 were measured in duplicate and interpolated from the MIP5 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP5 concentration was determined to be 1293 pg/mL in HT-29 cell extract and 685.5 pg/mL in colon tissue extract.
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Serum from nine individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP5 concentration was determined to be 7978 pg/mL with a range of 3361 – 13894 pg/mL.
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MIP5 is increased in serum of patients with rheumatoid arthritis.Serum was pooled from fifty apparently healthy donors and compared to serum from a patient who tested positive for rheumatoid factor (RF value 406.2 IU/mL). The concentrations of MIP5 were measured from serum samples in 3 different dilutions in duplicates and interpolated from the MIP5 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean MIP5 concentration was determined to be 8753 pg/mL in healthy human serum and 26193 pg/mL in rheumatoid arthritis patient serum.
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Sandwich ELISA - Human MIP5 ELISA Kit (ab217774)To learn more about the advantages of recombinant antibodies see here.
