Human MIP-3 alpha ELISA Kit (CCL20) (ab269562)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.11 pg/ml
- Range: 18.75 pg/ml - 1200 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MIP-3 alpha ELISA Kit (CCL20)
See all Macrophage Inflammatory Protein 3 alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 3% Inter-assay Sample n Mean SD CV% Supernatant 3 3.1% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
5.11 pg/ml -
Range
18.75 pg/ml - 1200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 92 84% - 104% Serum 86 83% - 90% Hep Plasma 98 88% - 106% EDTA Plasma 88 83% - 90% Cit plasma 96 84% - 93% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human MIP-3 alpha ELISA Kit (CCL20) (ab269562) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIP-3 alpha (CCL20) protein in cell culture supernatant, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MIP-3 alpha (CCL20) with 5.11 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
MIP-3 alpha (MIP3a, CCL20) is a CC chemokine with a selective chemotactic activity for lymphocytes and dendritic cells. Its main function is to inhibit proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3 alpha is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. There are also low levels in thymus, prostate, testis, small intestine and colon. MIP-3 alpha is induced by bacterial lipopolysaccharides, TNF and IFNG/IFN-gamma. Stimulation with phorbol myristate acetate (PMA) in U-937 cell line and Bowes melanoma will induce expression. MIP-3 alpha shares 64.2%, 60.8%, 69.8%, 86.5% sequence homology with mouse, rat, cow, and monkey, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human MIP-3 alpha (CCL20) Capture Antibody 1 x 600µl 10X Human MIP-3 alpha (CCL20) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human MIP-3 alpha (CCL20) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. -
Tissue specificity
Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsC-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Beta chemokine exodus 1
- Beta-chemokine exodus-1
- C C motif chemokine ligand 20
see all -
Database links
- Entrez Gene: 6364 Human
- Omim: 601960 Human
- SwissProt: P78556 Human
- Unigene: 75498 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human MIP-3 standard curve comparisonStandard Curve comparison between the original human MMP3 SimpleStep ELISA kit (ab178015) and current human MMP3 SimpleStep ELISA kit (ab269562).
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Example of human MIP-3 alpha standard curve in Sample Diluent NS.The MIP-3 alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MIP-3 alpha in human cell culture supernatant samples.The concentrations of MIP-3 alpha were measured in duplicates, interpolated from the MIP-3 alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated PBMC 100%, 1.5% PHA stimulated PBMC cell culture supernatant 50%, unstimulated THP-1 100%, and 1ug/mL IFN-gamma and 1ug/mL LPS stimulated THP-1 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP-3 alpha concentration was determined to be undetectable in unstimulated PBMC supernatant, 1614.14 pg/mL in PHA stimulated PBMC, undetectable in unstimulated THP-1 supernatant, and 1209.37 pg/mL in IFN-gamma and LPS stimulated THP-1 supernatant.
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Sandwich ELISA - Human MIP-3 alpha ELISA Kit (CCL20) (ab269562)To learn more about the advantages of recombinant antibodies see here.
