Human MEPE ELISA Kit (ab277405)
Key features and details
- Sensitivity: 8.4 ng/ml
- Range: 8.192 ng/ml - 8000 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MEPE ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% General Inter-assay Sample n Mean SD CV% General -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8.4 ng/ml -
Range
8.192 ng/ml - 8000 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 80.13 69% - 95% Serum 87.11 69% - 103% Plasma 98.58 93% - 105% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human MEPE ELISA Kit (ab277405) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MEPE in serum, plasma (collect plasma using EDTA and Citrate as an anticoagulant; Heparin is not recommended for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Human MEPE coated on a 96-well plate. Standards and samples are pipetted into the wells and Human MEPE present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human MEPE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human MEPE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 150X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human MEPE Antibody 2 vials Anti-Human MEPE coated Microplate 1 unit Human MEPE standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Seems to play a role in mineralization. -
Tissue specificity
Expressed by osteoblasts. Secreted from oncogenic hypophosphataemic tumors. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- Matrix extracellular phosphoglycoprotein
- MEPE
- MEPE_HUMAN
see all -
Database links
- Entrez Gene: 56955 Human
- Omim: 605912 Human
- SwissProt: Q9NQ76 Human
- Unigene: 676357 Human
Images
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Human MEPE ELISA kit (ab277405) Standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.