Human MELK knockout HCT116 cell lysate (ab257537)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab263566 - Human MELK knockout HCT116 cell lysate 1 x 100µg ab255555 - Human wild-type HCT116 cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X D5S818: 10, 11 D13S317: 10, 12 D7S820: 11, 12 D16S539: 11, 13 vWA: 17, 22 TH01: 8,9 TPOX: 8, 9 CSF1PO: 7, 10
Images
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Western blot - Human MELK knockout HCT116 cell lysate (ab257537)
Lane 1: Wild-type HCT116 cell lysate 20 ug
Lane 2: MELK knockout HCT116 cell lysate 20 ug
Lanes 1 - 2: Merged signal (red and green). Green - ab108529 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab108529 was shown to react with MELK in wild-type HCT116 cells in western blot with loss of signal observed in MELK knockout cell line ab266896 (MELK knockout cell lysate ab257537). Wild-type and MELK knockout HCT116 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108529 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human MELK knockout HCT116 cell lysate (ab257537)Allele-1: 1 bp deletion in exon9
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Sanger Sequencing - Human MELK knockout HCT116 cell lysate (ab257537)Allele-2: Insertion of the selection cassette in exon9