Lane 1: Wild-type HEK293T cell lysate (20 ug)
Lane 2: MDH2 knockout HEK293T cell lysate (20 ug)
Lane 3: K-562 cell lysate (20 ug)
Lane 4: Human eyeball tissue lysate (20 ug)

ab181857 was shown to specifically react with MDH2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266449 (knockout cell lysate ab257533) was used. Wild-type and MDH2 knockout samples were subjected to SDS-PAGE. ab181857 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4^oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

Lane 1: Wild-type HEK293T cell lysate (20 ug)
Lane 2: MDH2 knockout HEK293T cell lysate (20 ug)
Lane 3: K-562 cell lysate (20 ug)
Lane 4: Human eyeball tissue lysate (20 ug)

ab181873 was shown to specifically react with MDH2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266449 (knockout cell lysate ab257533) was used. Wild-type and MDH2 knockout samples were subjected to SDS-PAGE. ab181873 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4^oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

Allele-1: 4 bp deletion in exon 4

Allele-2: Insertion of the selection cassette in exon 4