Human MD1 ELISA Kit (ab277441)
Key features and details
- Sensitivity: 0.43 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MD1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.43 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 80.7 72% - 88% Plasma 82.62 76% - 88% Cell culture media 83.64 69% - 94% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human MD1 ELISA Kit (ab277441) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MD1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human MD1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human MD1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human MD1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human MD1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human MD1 Antibody 2 vials Anti-Human MD1 coated Microplate 1 unit Human MD1 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
May cooperate with CD180 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) and cytokine production. Important for efficient CD180 cell surface expression. -
Tissue specificity
Highly expressed in B-cells, monocytes and tonsil. -
Cellular localization
Secreted > extracellular space. Associated with CD180 at the cell surface. - Information by UniProt
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Alternative names
- BPAD
- dJ80N2.1
- Ly 86
see all -
Database links
- Entrez Gene: 9450 Human
- Omim: 605241 Human
- SwissProt: O95711 Human
- Unigene: 653138 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.
