Human MCP4 ELISA Kit (ab239426)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.2 pg/ml
- Range: 3.9 pg/ml - 250 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MCP4 ELISA Kit
See all MCP4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% plasma 5 5.6% Inter-assay Sample n Mean SD CV% plasma 3 6.6% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.2 pg/ml -
Range
3.9 pg/ml - 250 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 91 88% - 95% Cell culture media 95 92% - 97% EDTA Plasma 95 90% - 99% Cit plasma 96 89% - 99% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human MCP4 ELISA Kit (ab239426) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP4 protein in edta plasma, serum, cell culture supernatant, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MCP4 with 1.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human MCP4 Capture Antibody 1 x 600µl 10X Human MCP4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human MCP4 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. -
Tissue specificity
Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsOne major form (form long), and two minor forms (short chain and medium chain) are produced by differential signal peptide cleavage. The medium chain is about 30-fold less active than the long chain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-C motif chemokine 13
- CCL13
- CCL13_HUMAN
see all -
Database links
- Entrez Gene: 6357 Human
- Omim: 601391 Human
- SwissProt: Q99616 Human
- Unigene: 414629 Human
Images
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Other - Human MCP4 ELISA Kit (ab239426)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human MCP4 standard curve in Sample Diluent NS.The MCP4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native MCP4 in human serum, plasma (citrate), and plasma (EDTA) samples.The concentrations of MCP4 were measured in duplicates, interpolated from the MCP4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP4 concentration was determined to be 35.0 pg/mL in serum, 56.2 pg/mL in plasma (citrate), and 36.6 pg/mL in plasma (EDTA). -
Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP4 concentration was determined to be 30.6 pg/mL with a range of 17.9 - 75.0 pg/mL.
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Sandwich ELISA - Human MCP4 ELISA Kit (ab239426)To learn more about the advantages of recombinant antibodies see here.