Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human MCP3 ELISA Kit (CCL7) (ab193769)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 10.3 pg/ml
  • Range: 46.875 pg/ml - 3000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human MCP3 ELISA Kit (CCL7)
    See all MCP3 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 2%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 6.8%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    10.3 pg/ml

  • Range

    46.875 pg/ml - 3000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 99.7 98.4% - 101.2%
    Cell culture media 107.3 103.8% - 112.9%
    Hep Plasma 98.5 97% - 99.5%
    EDTA Plasma 87.8 84.7% - 89.6%
    Cit plasma 99 95.6% - 101%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human MCP3 ELISA Kit (CCL7) (ab193769) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP3 (CCL7) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MCP3 (CCL7) with 10.3 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    MCP3 (CCL7) is a monomeric secreted chemotactic factor of intercrine (chemokine cc) family. MCP3 attracts monocytes and eosinophils, but not neutrophils. MCP3 augments monocyte anti-tumor activity. It also induces the release of gelatinase B. MCP3 protein can bind heparin. Binds to CCR1, CCR2 and CCR3.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - Human MCP3 ELISA Kit (CCL7) (ab193769)
    Other - Human MCP3 ELISA Kit (CCL7) (ab193769)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of MCP3 standard curve prepared in Sample Diluent NS.
    Example of MCP3 standard curve prepared in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Comparison of MCP3 concentrations in unstimulated and PHA-stimulated Human PBMC.
    Comparison of MCP3 concentrations in unstimulated and PHA-stimulated Human PBMC.

    PBMC were grown in the absence or presence of phytohemagglutinin (PHA) for 48 hours. MCP3 concentrations were measured in 4 X and 8 X diluted cell culture supernatants of the unstimulated PBMC and the stimulated PBMC, and 10F RPMI1640 media. Raw data values (mean +/-SD, n=2) are graphed. The dotted line represents Blank control.

  • Example of MCP3 standard curve prepared in Sample Diluent 50 BP.
    Example of MCP3 standard curve prepared in Sample Diluent 50 BP.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Titration of 48 hours PHA-stimulated PBMC cell culture supernatant samples within the working range of the assay.
    Titration of 48 hours PHA-stimulated PBMC cell culture supernatant samples within the working range of the assay.

    Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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