Human MATN3/Matrilin-3 ELISA Kit (ab277416)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 20.48 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MATN3/Matrilin-3 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
20 pg/ml -
Range
20.48 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 129 117% - 148% Plasma 97.84 71% - 124% Cell culture media 112.5 101% - 125% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human MATN3/Matrilin-3 ELISA Kit (ab277416) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MATN3/Matrilin-3 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human MATN3/Matrilin-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human MATN3/Matrilin-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human MATN3/Matrilin-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human MATN3/Matrilin-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human MATN3/Matrili-3 Antibody 2 vials Anti-Human MATN3 Matrilin-3 coated Microplate 1 unit Human MATN3/Matrilin-3 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Major component of the extracellular matrix of cartilage and may play a role in the formation of extracellular filamentous networks. -
Tissue specificity
Expressed only in cartilaginous tissues, such as vertebrae, ribs and shoulders. -
Involvement in disease
Multiple epiphyseal dysplasia 5
Spondyloepimetaphyseal dysplasia MATN3-related
Osteoarthritis 2 -
Sequence similarities
Contains 4 EGF-like domains.
Contains 1 VWFA domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AV009181
- DIPOA
- EDM5
see all -
Database links
- Entrez Gene: 4148 Human
- Omim: 602109 Human
- SwissProt: O15232 Human
- Unigene: 656199 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.