Human MAPK3 (ERK1) knockout HEK-293T cell lysate (ab257099)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260942 - Human MAPK3 knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human MAPK3 (ERK1) knockout HEK293T cell lysate (ab257099)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: MAPK3 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab109282 observed at 43 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109282 Anti-ERK1 antibody [EP4967] was shown to specifically react with ERK1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266519 (knockout cell lysate ab257099) was used. Wild-type and ERK1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109282 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human MAPK3 (ERK1) knockout HEK293T cell lysate (ab257099)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: MAPK3 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab32537 observed at 43 kDa. Red - loading control ab8245 observed at 37 kDa.
ab32537 Anti-ERK1 antibody [Y72] was shown to specifically react with ERK1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266519 (knockout cell lysate ab257099) was used. Wild-type and ERK1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32537 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human MAPK3 knockout HEK293T cell lysate (ab257099)Homozygous: 1 bp deletion in exon1
