Human MAPK14 (p38) knockout HEK-293T cell lysate (ab263787)
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab255510 - Human MAPK14 knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
-
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
-
Western blot - Human MAPK14 knockout HEK293T cell lysate (ab263787)
Lane 1: HeLa cell lysate (20 µg)
Lane 2: Jurkat cell lysate (20 µg)
Lane 3: Wild-type HEK-293TT cell lysate (20 µg)
Lane 4: MAPK14 knockout HEK-293TT cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab182453 observed at 40 kDa. Red - loading control, ab130007 observed at 125 kDa.
ab182453 was shown to react with p38 in HEK-293TT wildtype. Loss of signal was observed when knockout cell line ab255406 (knockout cell lysate ab263787) was used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab182453 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Western blot - Human MAPK14 knockout HEK293T cell lysate (ab263787)Lane 1: HeLa cell lysate (20 µg)
Lane 2: Jurkat cell lysate (20 µg)
Lane 3: Wild-type HEK-293T cell lysate (20 µg)
Lane 4: MAPK14 knockout HEK-293T cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32142 observed at 40 kDa. Red - loading control, ab130007 observed at 125 kDa.
ab32142 was shown to react with p38 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255406 (knockout cell lysate ab263787) was used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab32142 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Sanger Sequencing - Human MAPK14 knockout HEK293T cell lysate (ab263787)Allele-1: 1 bp insertion in exon 1; Allele-2: 11 bp deletion in exon 1