Human MAPK13 ELISA Kit (ab234562)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 38.4 pg/ml
- Range: 141 pg/ml - 9000 pg/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MAPK13 ELISA Kit
See all p38 delta/MAPK13 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell extract 5 4.6% Inter-assay Sample n Mean SD CV% Cell extract 3 5.5% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
38.4 pg/ml -
Range
141 pg/ml - 9000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 119 117% - 124% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human MAPK13 ELISA Kit (ab234562) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MAPK13 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MAPK13 with 38.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human MAPK13 Capture Antibody 1 x 600µl 10X Human MAPK13 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent BAR 1 x 6ml Human MAPK13 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. -
Tissue specificity
Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme. - Information by UniProt
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Alternative names
- MAP kinase 13
- MAP kinase p38 delta
- MAPK 13
see all -
Database links
- Entrez Gene: 5603 Human
- Omim: 602899 Human
- SwissProt: O15264 Human
- Unigene: 178695 Human
Images
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Other - Human MAPK13 ELISA Kit (ab234562)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human MAPK13 standard curve in 1X Cell Extraction Buffer PTR.The MAPK13 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MAPK13 in human HEK293T and A431 cell extract samples based on a 500 µg/mL extract load.The concentrations of MAPK13 were measured in duplicate and interpolated from the MAPK13 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MAPK13 concentration was determined to be 9009 pg/mL in HEK293T and 8141 pg/mL in A431 cell extract samples.
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Sandwich ELISA - Human MAPK13 ELISA Kit (ab234562)To learn more about the advantages of recombinant antibodies see here.