Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human LRG ELISA Kit (LRG1) (ab260066)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 11.08 pg/ml
  • Range: 46.88 pg/ml - 3000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human LRG ELISA Kit (LRG1)
    See all LRG1/LRG kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 5.4%

  • Sample type

    Cell culture supernatant, Serum, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    11.08 pg/ml

  • Range

    46.88 pg/ml - 3000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 86 % - %
    Serum 92 % - %
    Cit plasma 95 % - %

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human
    Does not react with: Cow

  • Product overview

    Human LRG ELISA Kit (LRG1) (ab260066) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LRG (LRG1) protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LRG (LRG1) with 11.08 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    LRG (LRG1) is a leucine-rich repeat glycoprotein present in plasma during granulocyte differentiation. LRG is involved in neovascularization by modulation of TGFbeta signaling in endothelial cells. Elevated levels of LRG are seen in inflammation and in rheumatic diseases like rheumatoid arthritis, adult-onset Still’s disease, and Kawaski disease.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of human LRG standard curve in Sample Diluent NS.
    Example of human LRG standard curve in Sample Diluent NS.

    The LRG standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native LRG in human serum, plasma (citrate), and U937 cell culture supernatant samples.
    Interpolated concentrations of native LRG in human serum, plasma (citrate), and U937 cell culture supernatant samples.

    The concentrations of LRG were measured in duplicates, interpolated from the LRG standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 10,000 and plasma (citrate) 1: 5,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LRG concentration was determined to be 17.50 µg/mL in serum and 10.52 µg/mL in plasma (citrate).

  • Sandwich ELISA - Human LRG ELISA Kit (LRG1) (ab260066)
    Sandwich ELISA - Human LRG ELISA Kit (LRG1) (ab260066)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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