Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human HAI-1 ELISA Kit (ab267639)

Key features and details

  • Sensitivity: 0.8 ng/ml
  • Range: 0.819 ng/ml - 200 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human HAI-1 ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.8 ng/ml

  • Range

    0.819 ng/ml - 200 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 72.47 68% - 82%
    Plasma 100 69% - 150%
    Cell culture media 72.35 67% - 83%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human HAI-1 ELISA Kit is designed for the quantitative determination of HAI-1 in cell culture supernatants, plasma and serum samples.


    Δ Note: Human HAI-1 concentration is low in normal serum and plasma and may not be detectable in this assay.


    This assay employs an antibody specific for human HAI-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and HAI-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human HAI-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HAI-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    No cross-reactivity with mouse HAI-1, human APP, GASP-1, GASP-2, HAI-2A, TFPI, TFPI-2 is observed.


     

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Example data
    Example data

    This standard curve is for demonstration only. A standard curve must be run with each assay.

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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