Human Liver-FABP ELISA Kit (ab218261)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 9.4 pg/ml
- Range: 14.8 pg/ml - 950 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Liver-FABP ELISA Kit
See all liver FABP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 6.2% Inter-assay Sample n Mean SD CV% Overall 3 4.2% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
9.4 pg/ml -
Range
14.8 pg/ml - 950 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 103 97% - 113% Urine 104 101% - 105% Serum 97 93% - 101% Hep Plasma 85 81% - 92% EDTA Plasma 93 87% - 98% Cit plasma 88 82% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Liver-FABP ELISA Kit (ab218261) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Liver-FABP protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Liver-FABP with 9.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Liver-FABP is a 14 kDa member of the FABP family. Liver-FABP binds and transports cholesterol, free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules. A variety of cells express Liver-FABP, including hepatocytes, simple columnar epithelium of the small intestine, and proximal tubular epithelium of the kidney.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Liver-FABP Capture Antibody 1 x 600µl 10X Human Liver-FABP Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human L-FABP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- FABP 1
- FABP1
- FABPL
see all -
Database links
- Entrez Gene: 2168 Human
- Omim: 134650 Human
- SwissProt: P07148 Human
- Unigene: 380135 Human
Images
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Other - Human Liver-FABP ELISA Kit (ab218261)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Human Liver-FABP standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Liver-FABP in human serum, plasma, urine, and cell culture supernatant samples.The concentrations of Liver-FABP were measured in duplicates, interpolated from the Liver-FABP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 7.5%, plasma (citrate) 7.5%, plasma (heparin) 7.5%, plasma EDTA) 7.5%, urine 50 %, and spleen HepG2 supernatant 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Liver-FABP concentration was determined to be 11.2 ng/mL in serum, 12.2 ng/mL in plasma (citrate) and 13.6 ng/mL in plasma (heparin), 11.0 ng/mL in plasma (EDTA), 0.682 ng/mL in urine, and 11.4 ng/mL in HepG2 supernatant.
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Liver-FABP concentration was determined to be 10.3 ng/mL with a range of 6.36 – 19.0 ng/mL.
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Sandwich ELISA - Human Liver-FABP ELISA Kit (ab218261)To learn more about the advantages of recombinant antibodies see here.
