Human LAIR2 ELISA Kit (ab277432)
Key features and details
- Sensitivity: 2.2 ng/ml
- Range: 1.966 ng/ml - 480 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human LAIR2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.2 ng/ml -
Range
1.966 ng/ml - 480 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 93.31 68% - 146% Plasma 90.25 69% - 115% Cell culture media 138.1 134% - 143% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human LAIR2 ELISA Kit (ab277432) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LAIR2 in serum (human LAIR2 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Human LAIR2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human LAIR2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human LAIR2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human LAIR2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human LAIR2 Antibody 2 vials Anti-Human LAIR2 coated Microplate 1 unit Human LAIR2 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CD306
- CD306 antigen
- LAIR 2
see all -
Database links
- Entrez Gene: 3904 Human
- Omim: 602993 Human
- SwissProt: Q6ISS4 Human
- Unigene: 43803 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.