Human IL7 knockout A549 cell pellet (ab279157)
Overview
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Product name
Human IL7 knockout A549 cell pellet
See all IL-7 kits -
Product overview
Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.
Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation. -
Parental Cell Line
A549 -
Organism
Human -
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 25 bp deletion in exon2. -
Passage number
Knockout validation
Sanger SequencingNotes
Pellet size: 5 million cells/vial.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human IL7 knockout A549 cell pellet 1 vial Human wild-type A549 cell pellet 1 vial -
Research areas
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Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12
Target
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Function
Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. -
Sequence similarities
Belongs to the IL-7/IL-9 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IL 7
- IL-7
- Il7
see all
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human IL7 knockout A549 cell pellet 1 vial Human wild-type A549 cell pellet 1 vial -
Research areas
-
Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12
Images
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Sanger Sequencing - Human IL7 knockout A549 cell pellet (ab279157)
Allele-1: 25 bp deletion in exon2
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Sanger Sequencing - Human IL7 knockout A549 cell pellet (ab279157)Allele-2: 1 bp insertion in exon2