Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (ab251120)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16530] to IRAK2 - BSA and Azide free
  • Suitable for: IP, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-IRAK2 antibody [EPR16530] - BSA and Azide free
    See all IRAK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR16530] to IRAK2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251120 is the carrier-free version of ab192249. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251120 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (ab251120)
    Western blot - Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (ab251120)
    All lanes : Anti-IRAK2 antibody [EPR16530] (ab192249) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : MOLT-4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 69 kDa



    This data was developed using ab192249, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (ab251120)
    Flow Cytometry - Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (ab251120)
    This data was developed using ab192249, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed MOLT4 cells labeling IRAK2 with ab192249 at 1/220 dilution (pink), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
  • Western blot - Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (ab251120)
    Western blot - Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (ab251120)
    All lanes : Anti-IRAK2 antibody [EPR16530] (ab192249) at 1/1000 dilution

    Lane 1 : THP1 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 69 kDa



    This data was developed using ab192249, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (ab251120)
    Immunoprecipitation - Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (ab251120)
    This data was developed using ab192249, the same antibody clone in a different buffer formulation.Western blot analysis of IRAK2 in MOLT4 cell lysates immunoprecipitated using ab192249 at 1/60 dilution. Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.Blocking/Dilution buffer: 5% NFDM /TBST.
  • Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (ab251120)
    Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (ab251120)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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