Human IL6ST knockout A549 cell pellet (ab279224)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human IL6ST knockout A549 cell pellet 1 vial Human wild-type A549 cell pellet 1 vial -
Research areas
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Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12
Images
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Western blot - Human IL6ST knockout A549 cell pellet (ab279224)
Lane 1: Wild-type A549 cell lysate (20µg)
Lane 2: IL6ST knockout A549 cell lysate (20µg)
Lane 3: PC3 cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab217671 observed at 130 kDa. Red - loading control ab8245 observed at 37 kDa.
ab217671 Anti-CD130 (gp130) antibody [EPR21732] was shown to specifically react with CD130 (gp130) in wild-type A549 cells in western blot. Loss of signal was observed when knockout cell line ab266939 (knockout cell lysate ab257208) was used. Wild-type and CD130 (gp130) knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab217671 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human IL6ST knockout A549 cell pellet (ab279224)Homozygous: 1 bp insertion in exon4
