Human IL-6 Receptor ELISA Kit (ab181424)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 46 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-6 Receptor ELISA Kit
See all IL-6R kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human Plasma 6 1.7% Inter-assay Sample n Mean SD CV% Human Plasma 3 6.2% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
46 pg/ml -
Range
156 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 93 92% - 94% Serum 103 98% - 112% Cell culture media 98 96% - 101% Hep Plasma 97 94% - 101% EDTA Plasma 91 82% - 99% Cit plasma 98 94% - 104% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-6 Receptor (Interleukin-6 Receptor) ELISA kit (ab181424) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-6 Receptor protein in human serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human IL-6 Receptor with 46 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
Notes
Interleukin-6 Receptor (IL-6R, IL-6 Receptor), also known as Interleukin-6 Receptor subunit alpha and membrane glycoprotein 80, is part of a receptor that binds Interleukin-6 (IL-6). IL-6 Receptor weakly binds IL-6 with a low affinity, and then is presented to gp130 to facilitate transition into a high affinity signaling competent hexamer. Activation may lead to regulation of the immune response, acute-phase reactions and hematopoiesis. The production of IL-6 Receptor has been implicated in the pathogenesis of many diseases, including multiple myeloma, auto immune diseases and prostate cancer.IL-6 Receptor has two isoforms. The first is a transmembrane protein in which the N-terminal extracellular portion can be released by proteolytic cleavage. The second isoform is a secreted form that lacks the transmembrane and intracellular portions. This assay is directed at the extracellular portion of IL-6 Receptor and detects both the soluble and membrane bound forms.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-6 Receptor Capture Antibody 1 x 600µl 10X Human IL-6 Receptor Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human IL-6 Receptor Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity. -
Tissue specificity
Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Domain
The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. -
Post-translational
modificationsA short soluble form may also be released from the membrane by proteolysis. -
Cellular localization
Secreted and Basolateral cell membrane. - Information by UniProt
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Alternative names
- CD 126
- CD126
- CD126 antigen
see all -
Database links
- Entrez Gene: 3570 Human
- Omim: 147880 Human
- SwissProt: P08887 Human
- Unigene: 709210 Human
- Unigene: 135087 Human
Images
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Other - Human IL-6 Receptor ELISA Kit (ab181424)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of IL-6 Receptor standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of IL-6 Receptor in Human serum and plasma titrations.The concentrations of IL-6 Receptor were interpolated from the IL-6 Receptor standard curve and corrected for sample dilution.
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Interpolated concentrations of IL-6 Receptor in Human serum and Human plasma.The concentrations of IL-6 Receptor were interpolated the IL-6 Receptor standard curve and corrected for sample dilution. The mean IL-6 Receptor concentration was determined to be 27,796 pg/mL in plasma (EDTA) and 25,731 pg/mL in serum.
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Interpolated concentrations of IL-6 Receptor in Human serum from 10 donors.Serum from 10 apparently healthy male donors was measured in duplicate for IL-6 Receptor. The mean IL-6 Receptor concentration was determined to be 24,806 pg/mL with a range of 17,960 – 35,807 pg/mL.
