Human IL-32 alpha ELISA Kit (ab267616)
Key features and details
- Sensitivity: 4.1 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-32 alpha ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
4.1 pg/ml -
Range
4.1 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 113.4 102% - 123% Plasma 112.9 101% - 119% Cell culture media 83.07 79% - 101% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-32 alpha ELISA Kit is designed for the quantitative determination of IL-32 alpha in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human IL-32 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-32 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-32 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-32 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Human IL-32 alpha coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human IL-32 alpha Detection Antibody 2 vials Human IL-32 alpha Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. -
Tissue specificity
Selectively expressed in lymphocytes. Expression more prominent in immunce cells than in non-immune cells. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IL 32alpha
- IL 32beta
- IL 32delta
see all -
Database links
- Entrez Gene: 9235 Human
- Omim: 606001 Human
- SwissProt: P24001 Human
- Unigene: 943 Human
Images
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Example standard curve
These standard curves are for demonstration only. A standard curve must be run with each assay.