Human IL-29 ELISA Kit (ab236715)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.8 pg/ml
- Range: 31.3 pg/ml - 3200 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-29 ELISA Kit
See all IL-29 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 2.8% Inter-assay Sample n Mean SD CV% Serum 3 2.5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7.8 pg/ml -
Range
31.3 pg/ml - 3200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 99 95% - 102% Serum 111 107% - 115% Cell culture media 106 102% - 109% Hep Plasma 117 116% - 117% EDTA Plasma 93 85% - 102% Cit plasma 88 85% - 91% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-29 ELISA Kit (ab236715) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-29 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-29 with 7.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-29 Capture Antibody 1 x 600µl 10X Human IL-29 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human IL-29 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine with immunomodulatory activity. May play a role in antiviral immunity. Up-regulates MHC class I antigen expression. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. -
Sequence similarities
Belongs to the IL-28/IL-29 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Cytokine ZCYTO21
- IFN lambda 1
- IFN-lambda-1
see all -
Database links
- Entrez Gene: 282618 Human
- Omim: 607403 Human
- SwissProt: Q8IU54 Human
- Unigene: 406745 Human
Images
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Other - Human IL-29 ELISA Kit (ab236715)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human IL-29 standard curve in Sample Diluent 10BP.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human IL-29 standard curve in Sample Diluent 50BP.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-29 in IFN-alpha and Poly(I:C) stimulated HUVEC cell culture supernatant samples.The concentrations of IL-29 were measured in duplicates, interpolated from the IL-29 standard curves and corrected for sample dilution. Undiluted samples are as follows: 50% HUVEC supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-29 concentration was determined to be 412.4 pg/mL in neat stimulated HUVEC cell culture supernatant.
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Comparison of IL-29 in unstimulated and IFN alpha and Poly(I:C) stimulated HUVEC cell culture supernatants.HUVEC cells were cultured for 20 hours in the presence or absence of 100 IU/mL human IFN alpha and 30 µg/mL Poly(I:C). The concentrations of IL-29 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the IL-29 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean IL-29 concentration was determined to be 412.4 pg/mL in stimulated HUVEC cell culture supernatant, and undetectable in unstimulated HUVEC cell culture supernatant and RPMI1640 media containing 10% FBS (not shown).
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Sandwich ELISA - Human IL-29 ELISA Kit (ab236715)To learn more about the advantages of recombinant antibodies see here.
