Human IL-24 ELISA Kit (ab267595)
Key features and details
- Sensitivity: 0.41 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-24 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.41 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 73.92 69% - 75% Plasma 73 67% - 85% Cell culture media 129.8 108% - 149% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-24 ELISA Kit is designed for the quantitative determination of IL-24 in cell culture supernatants, plasma and serum samples.
Δ Note: Human IL-24 concentration is low in normal serum/plasma, and may not be detectable in this assay. Collect plasma using EDTA or Citrate as an anticoagulant. Heparin is not recommended.
This assay employs an antibody specific for human IL-24 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-24 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-24 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-24 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Less than 0.2% cross-reactivity with mouse IL-24 is observed.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Anti-Human IL-24 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Human IL-24 Detection Antibody 2 vials Human IL-24 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. -
Tissue specificity
Up-regulated in melanoma cells induced to terminally differentiate. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C49A
- FISP
- IL 24
see all -
Database links
- Entrez Gene: 11009 Human
- Omim: 604136 Human
- SwissProt: Q13007 Human
- Unigene: 58831 Human
- Unigene: 728324 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.