Human IL-2 ELISA Set (without plates) (ab48471)
Overview
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Product name
Human IL-2 ELISA Set (without plates)
See all IL-2 kits -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
1000 pg/ml - 31.25 pg/mlAssay duration
Multiple steps standard assaySpecies reactivity
Reacts with: HumanProduct overview
Abcam’s IL-2 Human in vitro ELISA Set is designed for the quantitative measurement of IL-2 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The IL-2 ELISA set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA set is intended to measure human IL-2 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Tested applications
Suitable for: Sandwich ELISAmore detailsPlatform
ReagentsProperties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-IL-2 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml IL-2 Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml IL-2 Standard (Lyophilized) Yellow 5 vials 10 vials 15 vials Ready-to-Use TMB 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials -
Research areas
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Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
Sequence similarities
Belongs to the IL-2 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Aldesleukin
- IL 2
- IL-2
see all -
Database links
- Entrez Gene: 3558 Human
- Omim: 147680 Human
- SwissProt: P60568 Human
- Unigene: 89679 Human
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-IL-2 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml IL-2 Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml IL-2 Standard (Lyophilized) Yellow 5 vials 10 vials 15 vials Ready-to-Use TMB 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials -
Research areas
-
Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
Sequence similarities
Belongs to the IL-2 family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- Aldesleukin
- IL 2
- IL-2
see all -
Database links
- Entrez Gene: 3558 Human
- Omim: 147680 Human
- SwissProt: P60568 Human
- Unigene: 89679 Human
Images
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IL-2 Human ELISA SetIL-2 Standard curve ranging from 31.25 to 1000 pg/mL