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Human IL-17B ELISA Kit (ab243670)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.6 pg/ml
  • Range: 18.8 pg/ml - 1200 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human IL-17B ELISA Kit
    See all IL-17B kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    serum 8 3.3%
    Inter-assay
    Sample n Mean SD CV%
    serum 3 5.6%

  • Sample type

    Cell culture supernatant, Serum, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    3.6 pg/ml

  • Range

    18.8 pg/ml - 1200 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 93 84% - 97%
    Cell culture media 92 87% - 97%
    EDTA Plasma 82 74% - 88%
    Cit plasma 86 78% - 92%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human IL-17B ELISA Kit (ab243670) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-17B protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-17B with 3.6 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IL-17B Capture Antibody 1 x 600µl
    10X Human IL-17B Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Human IL-17B Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1.

  • Tissue specificity

    Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.

  • Sequence similarities

    Belongs to the IL-17 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Cytokine like protein Zcyto7
    • Cytokine Zcyto7
    • IL 17B
    • IL 20
    • IL-17B
    • IL-20
    • IL17B
    • IL17B_HUMAN
    • IL20
    • Interleukin 17 beta
    • Interleukin 17B
    • Interleukin 20
    • Interleukin-17B
    • Interleukin-20
    • MGC138900
    • MGC138901
    • Neuronal interleukin 17 related factor
    • Neuronal interleukin-17-related factor
    • NIRF
    • ZCYTO7
    see all

  • Database links

    Images

    • Other - Human IL-17B ELISA Kit (ab243670)
      Other - Human IL-17B ELISA Kit (ab243670)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human IL-17B standard curve in Sample Diluent NS.
      Example of human IL-17B standard curve in Sample Diluent NS.

      The IL-17B standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of spike IL-17B in human serum, plasma and cell culture media (RPMI 1640 containing 10% FBS) samples.
      Interpolated concentrations of spike IL-17B in human serum, plasma and cell culture media (RPMI 1640 containing 10% FBS) samples.

      The concentrations of IL-17B were measured in duplicates, interpolated from the IL-17B standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and cell culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

       

    • Sandwich ELISA - Human IL-17B ELISA Kit (ab243670)
      Sandwich ELISA - Human IL-17B ELISA Kit (ab243670)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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