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Human IGFBP6 ELISA Kit (ab240686)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 4.03 pg/ml
  • Range: 7.81 pg/ml - 500 pg/ml
  • Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Cow, Human

Overview

  • Product name

    Human IGFBP6 ELISA Kit
    See all IGFBP6 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 3.3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 3.9%

  • Sample type

    Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    4.03 pg/ml

  • Range

    7.81 pg/ml - 500 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 99 91% - 106%
    Cell culture media 100 98% - 104%
    Hep Plasma 106 99% - 117%
    EDTA Plasma 99 96% - 102%
    Cit plasma 98 90% - 108%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Cow, Human

  • Product overview

    Human IGFBP6 ELISA Kit (ab240686) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP6 protein in cell culture media, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IGFBP6 with 4.03 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IGFBP6 Capture Antibody 1 x 600µl
    10X Human IGFBP6 Detector Antibody 2 vials
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Human IGFBP6 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

  • Sequence similarities

    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.

  • Post-translational
    modifications

    O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • IBP 6
    • IBP-6
    • IBP6
    • IBP6_HUMAN
    • IGF binding protein 6
    • IGF-binding protein 6
    • IGFBP 6
    • IGFBP-6
    • IGFBP6
    • Insulin like growth factor binding protein 6
    • Insulin-like growth factor-binding protein 6
    see all

  • Database links

    Images

    • Other - Human IGFBP6 ELISA Kit (ab240686)
      Other - Human IGFBP6 ELISA Kit (ab240686)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human IGFBP6 standard curve in Sample Diluent NS.
      Example of human IGFBP6 standard curve in Sample Diluent NS.
      The IGFBP6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
    • Interpolated concentrations of native IGFBP6 in human serum and plasma samples.
      Interpolated concentrations of native IGFBP6 in human serum and plasma samples.
      The concentrations of IGFBP6 were measured in duplicates, interpolated from the IGFBP6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 0.25%, plasma (citrate) 0.25%, plasma (EDTA) 0.25%, and plasma (heparin) 0.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP6 concentration was determined to be 362.78 ng/mL in serum, 276.59 ng/mL in plasma (citrate), 388.32 ng/mL plasma (EDTA), and 360.96 ng/mL plasma (Heparin).
    • Sandwich ELISA - Human IGFBP6 ELISA Kit (ab240686)
      Sandwich ELISA - Human IGFBP6 ELISA Kit (ab240686)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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