Human IGFBP2 ELISA Kit (ab272207)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 115.38 pg/ml
- Range: 312.5 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IGFBP2 ELISA Kit
See all IGFBP2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.7% Inter-assay Sample n Mean SD CV% Serum 3 3.2% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
115.38 pg/ml -
Range
312.5 pg/ml - 20000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 104 97% - 108% Urine 86 85% - 89% Serum 94 92% - 98% Hep Plasma 93 89% - 98% EDTA Plasma 94 91% - 97% Cit plasma 88 82% - 95% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IGFBP2 ELISA kit (ab272207) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP2 protein in serum, plasma, urine and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IGFBP2 with 115.38 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Insulin-like growth factor-binding protein 2 (IGFBP2) is an IGF binding protein involved in prolonging its half-life and has both inhibitory and stimulatory effects on IGFs. IGFBP2 can also alter the interaction of IGFs with their receptors.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests SimpleStep Pre-Coated 96-Well Microplate (ab206978) — Sample Diluent NS (ab193972) — Antibody Diluent 4BI — Human IGFBP2 Lyophilized Recombinant Protein — 10X Human IGFBP2 Capture Antibody — 10X Human IGFBP2 Detector Antibody — Plate Seals — 10X Wash Buffer PT (ab206977) — Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Domain
The C-terminus is required for IGF-binding and growth inhibition. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- BP 2
- BP2
- IBP 2
see all -
Database links
- Entrez Gene: 3485 Human
- Omim: 146731 Human
- SwissProt: P18065 Human
- Unigene: 438102 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human IGFBP2 competitor curve comparisonStandard Curve comparison between Human IGBPF2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor.
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Human IGFBP2 standard curve comparisonStandard Curve comparison between the original Human IGFBP2 SimpleStep ELISA kit (ab215082) and current Human MMP3 SimpleStep ELISA kit (ab272207).
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Example of human IGFBP2 standard curve in Sample Diluent NS.The IGFBP2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IGFBP2 in human serum and plasma samples.The concentrations of IGFBP2 were measured in duplicates, interpolated from the IGFBP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 5%, plasma (heparin) 10%, and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 257.87 ng/mL in serum, 273.81 ng/mL in plasma (citrate), 139.86 ng/ml in plasma (heparin), and 118.43 ng/ml in plasma (EDTA).
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Interpolated concentrations of native IGFBP2 in human PBMC cell culture supernatant samples treated for 46 hours with 1.5% PHA-M.The concentrations of IGFBP2 were measured in duplicates, interpolated from the IGFBP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 3,034.42 pg/mL in PBMC supernatant.
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Serum from ten individual healthy human male donors was measured in duplicate. -
Sandwich ELISA - Human IGFBP2 ELISA Kit (ab272207)To learn more about the advantages of recombinant antibodies see here.
