Human IgA Matched Antibody Pair Kit (ab219536)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 250 pg/ml - 16000 pg/ml
Overview
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Product name
Human IgA Matched Antibody Pair Kit
See all Human IgA kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
47 pg/ml -
Range
250 pg/ml - 16000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human IgA Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human IgA.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab196263. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human IgA Capture Antibody 2 x 50µg 1 x 50µg Human IgA Detector Antibody 2 x 12.5µg 1 x 12.5µg Human IgA Lyophilized Protein 2 vials 1 vial -
Research areas
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Relevance
Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. -
Cellular localization
Secreted -
Alternative names
- FLJ14473
- FLJ35065
- FLJ35500
see all -
Database links
- Entrez Gene: 3493 Human
- Entrez Gene: 3494 Human
- Omim: 147000 Human
- Omim: 146900 Human
- SwissProt: P01877 Human
- SwissProt: P01876 Human
Images
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Human IgA Standard Curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human IgA Matched Antibody Pair Kit (ab219536)To learn more about the advantages of recombinant antibodies see here.
