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Human HSP90AA1 (Hsp90 alpha) knockout HEK293T cell pellet (ab279019)

Overview

  • Product name

    Human HSP90AA1 (Hsp90 alpha) knockout HEK293T cell pellet
    See all Hsp90 alpha kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Molecular chaperone. Has ATPase activity.

  • Sequence similarities

    Belongs to the heat shock protein 90 family.

  • Post-translational
    modifications

    ISGylated.

  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Alternative names

    • EL52
    • epididymis luminal secretory protein 52
    • Heat shock 86 kDa
    • heat shock 90kD protein 1, alpha
    • Heat shock 90kD protein 1, alpha like 4
    • heat shock 90kD protein, alpha-like 4
    • Heat shock 90kDa protein 1 alpha
    • Heat shock protein 90kDa alpha (cytosolic) class A member 1
    • Heat shock protein HSP 90-alpha
    • HS90A_HUMAN
    • HSP 86
    • HSP86
    • Hsp89
    • HSP89A
    • Hsp90
    • HSP90A
    • HSP90AA1
    • HSP90ALPHA
    • HSP90N
    • HSPC1
    • HSPCA
    • HSPCAL1
    • HSPCAL4
    • HSPN
    • LAP 2
    • LAP2
    • lipopolysaccharide-associated protein 2
    • LPS-associated protein 2
    • Renal carcinoma antigen NY-REN-38
    see all

Properties

Images

  • Western blot - Human HSP90AA1 (Hsp90 alpha) knockout HEK293T cell pellet (ab279019)
    Western blot - Human HSP90AA1 (Hsp90 alpha) knockout HEK293T cell pellet (ab279019)

    Lane 1:Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
    Lane 2:HSP90AA1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
    Lane 3:HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (20 ug)
    Lane 4:HAP1 whole cell lyate (20 ug)

    ab59459 was shown to specifically react with Hsp90 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266591 (knockout cell lysate ab258458) was used. Wild-type and Hsp90 knockout samples were subjected to SDS-PAGE. ab59459 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Human HSP90AA1 (Hsp90 alpha) knockout HEK293T cell pellet (ab279019)
    Western blot - Human HSP90AA1 (Hsp90 alpha) knockout HEK293T cell pellet (ab279019)

    Lane 1:Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
    Lane 2:HSP90AA1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
    Lane 3:HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (20 ug)
    Lane 4:HAP1 whole cell lyate (20 ug)

    ab79849 was shown to specifically react with Hsp90 alpha in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266591 (knockout cell lysate ab258458) was used. Wild-type and Hsp90 alpha knockout samples were subjected to SDS-PAGE. ab79849 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human HSP90AA1 (Hsp90 alpha) knockout HEK293T cell pellet (ab279019)
    Sanger Sequencing - Human HSP90AA1 (Hsp90 alpha) knockout HEK293T cell pellet (ab279019)
    Homozygous: 1 bp insertion in exon3

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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