Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human HNRNPA1 knockout HEK293T cell pellet (ab278886)

Overview

  • Product name

    Human HNRNPA1 knockout HEK293T cell pellet
    See all hnRNP A1 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 4 bp insertion in exon 1.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    Human HNRNPA1 knockout HEK293T cell pellet 1 vial
    Human wild-type HEK293T cell pellet 1 vial

  • Research areas

  • Cell type

    epithelial

  • STR Analysis

    Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12

Target

  • Function

    Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.

  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.

  • Post-translational
    modifications

    Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
    Sumoylated.

  • Cellular localization

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
  • Information by UniProt

  • Alternative names

    • HNRNPA 1
    • Helix destabilizing protein
    • Helix-destabilizing protein
    • Heterogeneous nuclear ribonucleoprotein A1
    • Heterogeneous nuclear ribonucleoprotein A1B protein
    • Heterogeneous nuclear ribonucleoprotein B2 protein
    • Heterogeneous nuclear ribonucleoprotein core protein A1
    • hnRNP A1
    • hnRNP core protein A1
    • HNRNPA1
    • HNRPA1
    • MGC102835
    • Nuclear ribonucleoprotein particle A1 protein
    • ROA1_HUMAN
    • Single strand DNA binding protein UP1
    • Single strand RNA binding protein
    • Single-strand RNA-binding protein
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    Human HNRNPA1 knockout HEK293T cell pellet 1 vial
    Human wild-type HEK293T cell pellet 1 vial

  • Research areas

  • Cell type

    epithelial

  • STR Analysis

    Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12

Images

  • Western blot - Human HNRNPA1 knockout HEK293T cell pellet (ab278886)
    Western blot - Human HNRNPA1 knockout HEK293T cell pellet (ab278886)

    Lane 1: Wild-type HEK293T cell lysate (20 µg)

    Lane 2: HNRNPA1 knockout HEK293T cell lysate (20 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lanes 1-3: Merged signal (red and green). Green - ab177152 observed at 37 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab177152 Anti-hnRNP A1 antibody [EPR12768] was shown to specifically react with hnRNP A1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266193 (knockout cell lysate ab256942) was used. Wild-type and hnRNP A1 knockout samples were subjected to SDS-PAGE. ab177152 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human HNRNPA1 knockout HEK293T cell pellet (ab278886)
    Sanger Sequencing - Human HNRNPA1 knockout HEK293T cell pellet (ab278886)

    Allele-1: 1 bp insertion in exon 1

     

  • Sanger Sequencing - Human HNRNPA1 knockout HEK293T cell pellet (ab278886)
    Sanger Sequencing - Human HNRNPA1 knockout HEK293T cell pellet (ab278886)

    Allele-2: 4 bp insertion in exon 1

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up