Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human HIF-2-alpha Antibody Pair - BSA and Azide free (ab244045)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

Overview

  • Product name

    Human HIF-2-alpha Antibody Pair - BSA and Azide free
    See all HIF-2-alpha kits

  • Assay type

    ELISA set

  • Range

    31.3 pg/ml - 2000 pg/ml

  • Species reactivity

    Reacts with: Human

  • Product overview

    The Antibody Pair can be used to quantify Human HIF-2-alpha. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab227898), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human HIF-2-alpha Capture Antibody (unconjugated) 1 x 100µg
    Human HIF-2-alpha Detector Antibody (unconjugated) 1 x 100µg

  • Research areas

  • Function

    Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

  • Tissue specificity

    Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

  • Involvement in disease

    Defects in EPAS1 are the cause of erythrocytosis familial type 4 (ECYT4) [MIM:611783]. ECYT4 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin concentration and hematocrit, and normal platelet and leukocyte counts.

  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.

  • Post-translational
    modifications

    In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
    In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.
    Phosphorylated on multiple sites in the CTAD.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Alternative names

    • Basic helix loop helix PAS protein MOP2
    • Basic-helix-loop-helix-PAS protein MOP2
    • bHLHe73
    • Class E basic helix-loop-helix protein 73
    • ECYT4
    • Endothelial PAS domain containing protein 1
    • Endothelial pas domain protein 1
    • Endothelial PAS domain-containing protein 1
    • EPAS 1
    • EPAS-1
    • EPAS1
    • EPAS1_HUMAN
    • HIF 1 alpha like factor
    • HIF 2 alpha
    • HIF-1-alpha-like factor
    • HIF-2-alpha
    • HIF2-alpha
    • HIF2A
    • HLF
    • Hypoxia inducible factor 2 alpha
    • Hypoxia inducible factor 2 alpha subunit
    • Hypoxia-inducible factor 2-alpha
    • Member of PAS protein 2
    • Member of pas superfamily 2
    • MOP 2
    • MOP2
    • PAS domain-containing protein 2
    • PASD2
    see all

  • Database links

    Images

    • Sandwich ELISA - Human HIF-2-alpha Antibody Pair - BSA and Azide free (ab244045)
      Sandwich ELISA - Human HIF-2-alpha Antibody Pair - BSA and Azide free (ab244045)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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