Human HGF ELISA Kit (Hepatocyte Growth Factor) (ab275901)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 18 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell culture media, Cell Lysate, Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
Overview
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Product name
Human HGF ELISA Kit (Hepatocyte Growth Factor)
See all HGF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 6% Inter-assay Sample n Mean SD CV% Extract 3 3% -
Sample type
Serum, Cell Lysate, Cell culture media, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
18 pg/ml -
Range
78.13 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 92 86% - 97% Cell Lysate 81 77% - 84% Cell culture media 105 103% - 107% Hep Plasma 77 75% - 80% Cit plasma 82 81% - 83% -
Assay time
1h 30m -
Assay duration
One step assay -
Product overview
Human Hepatocyte Growth Factor ELISA kit (ab275901) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Hepatocyte Growth Factor protein in human serum, plasma, cell culture supernatant, cell and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Hepatocyte Growth Factor with 18 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Hepatocyte Growth Factor (HGF) is mitogen of parenchymal hepatocytes. HGF also acts as a growth factor and is expressed by a wide variety of tissue and cell types. HGF binds to the HGF- Receptor (MET), a receptor tyrosine kinase, to help regulate cell growth, cell motility and morphogenesis. Elevated circulating levels of HGF can be measured as prognostic marker of severity of hypertension and heart failure. HGF is secreted as a full-length inactive polypeptide, and then is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta chain which dimerize producing the active heterodimeric molecule.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human HGF (Hepatocyte Growth Factor) Capture Antibody 1 x 600ml 10X Human HGF (Hepatocyte Growth Factor) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Human HGF (Hepatocyte Growth Factor) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. -
Involvement in disease
Deafness, autosomal recessive, 39 -
Sequence similarities
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. - Information by UniProt
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Alternative names
- DFNB39
- F TCF
- Fibroblast derived tumor cytotoxic factor
see all
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human HGF competitor curve comparisonStandard Curve comparison between Human HGF SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human HGF standard curve in Sample Diluent 25BP.The HGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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. Example of human HGF standard curve in 1X Cell Extraction Buffer PTR. The HGF standard curve was prepared as described in Section 10.The HGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native HGF in neat human serum and plasma samples.The concentrations of HGF were measured in duplicates in neat samples, interpolated from the HGF standard curves and plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 129 pg/mL in neat serum, 154 pg/mL in neat plasma (citrate) and 158 pg/mL in neat plasma (heparin).
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Interpolated concentrations of native HGF in human A-204 cell and placenta tissue based on 100 µg/mL and 200 µg/mL extract loads, respectively.The concentrations of HGF were measured in duplicate and interpolated from the HGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 4,077 pg/mL in A-204 extract and 2,681 pg/mL in placenta extract.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 151 pg/mL with a range of undetectable to 242 pg/mL.
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Sandwich ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (ab275901)To learn more about the advantages of recombinant antibodies see here.
