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Human Hexokinase II ELISA Kit (ab219043)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 11.37 pg/ml
  • Range: 62.5 pg/ml - 4000 pg/ml
  • Sample type: Cell culture extracts, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Hexokinase II ELISA Kit
    See all Hexokinase II kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    HeLa extract 8 3%
    Inter-assay
    Sample n Mean SD CV%
    HeLa extract 3 5.7%

  • Sample type

    Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    11.37 pg/ml

  • Range

    62.5 pg/ml - 4000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 99 96% - 102%
    Cell culture extracts 92 89% - 102%
    Tissue Extracts 96 91% - 103%
    Hep Plasma 101 98% - 106%
    EDTA Plasma 102 97% - 106%
    Cit plasma 96 91% - 99%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Hexokinase II ELISA Kit (ab219043) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Hexokinase II protein in cell culture extracts, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Hexokinase II with 11.37 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Hexokinases are involved in the pathway of hexose metabolism, where they phosphorylate hexose to form hexose-6-phosphate. Phosphorylation of hexose restricts transport out of the cell, therefore retained intracellularly for metabolic processes including glycolysis and glycogen synthesis. Hexokinases also phosphorylate glucose to glucose-6-phosphate.

    There are four mammalian hexokinases. Hexokinase II, also known as muscle form hexokinase, localizes to the outer membrane of mitochondria and is present in adipose tissue, skeletal muscle, and heart. Hexokinase II comprises of two homologous halves with both the C-terminal and N-terminal portions being catalytically active (with the N-terminal half having higher activity than the C-terminal half). Human Hexokinase I, mouse Hexokinase II, and rat Hexokinase II share 73.8%, 94.2%, and 94.4% in homology in amino acid sequence with human Hexokinase II, respectively.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human Hexokinase II ELISA Kit (ab219043)
    Other - Human Hexokinase II ELISA Kit (ab219043)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human Hexokinase II standard curve in Sample Diluent NS.
    Example of human Hexokinase II standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Example of human Hexokinase II standard curve in 1X Cell Extraction Buffer PTR.
    Example of human Hexokinase II standard curve in 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of spiked Hexokinase II in human serum and plasma samples.
    Interpolated concentrations of spiked Hexokinase II in human serum and plasma samples.

    The concentrations of Hexokinase II were measured in duplicates, interpolated from the Hexokinase II standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hexokinase II concentration was determined to be 7,009.26 pg/mL in neat serum, 7,887.82 pg/mL in neat plasma (citrate), 8,052.65 pg/mL in neat plasma (EDTA), and 8,675.63 pg/mL in neat plasma (heparin).

  • Interpolated concentrations of native Hexokinase II in human placenta, HeLa, and Jurkat extract samples based on a 50 µg/mL extract load.
    Interpolated concentrations of native Hexokinase II in human placenta, HeLa, and Jurkat extract samples based on a 50 µg/mL extract load.

    The concentrations of Hexokinase II were measured in duplicate and interpolated from the Hexokinase II standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hexokinase II concentration was determined to be 1,175.25 pg/mL in placenta extract, 2,844.72 pg/mL in HeLa extract, and 2,577.06 pg/mL in Jurkat extract.

     

     

  • Interpolated concentrations of native Hexokinase II in human fetal brain, mouse brain, and rat brain extract samples based on 25 µg/mL, 100 µg/mL, and 12.5 µg/mL extract loads, respectively.
    Interpolated concentrations of native Hexokinase II in human fetal brain, mouse brain, and rat brain extract samples based on 25 µg/mL, 100 µg/mL, and 12.5 µg/mL extract loads, respectively.

    The concentrations of Hexokinase II were measured in duplicate and interpolated from the Hexokinase II standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hexokinase II concentration was determined to be 624.25 pg/mL in human fetal brain extract, 4,691.00 pg/mL in mouse brain extract, and 973.64 pg/mL in rat brain extract.

  • Sandwich ELISA - Human Hexokinase II ELISA Kit (ab219043)
    Sandwich ELISA - Human Hexokinase II ELISA Kit (ab219043)
    To learn more about the advantages of recombinant antibodies see here.

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