Human GSDMD ELISA Kit (ab272463)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.154 ng/ml
- Range: 0.78 ng/ml - 50 ng/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human GSDMD ELISA Kit
See all GSDMD kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 2.5% Inter-assay Sample n Mean SD CV% Extract 3 8.8% -
Sample type
Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
0.154 ng/ml -
Range
0.78 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell Lysate 103 95% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human GSDMD ELISA kit (ab272463) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human GSDMD protein in cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate GSDMD with 0.154 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
GSDMD is a part of a family of highly conserved proteins expressed primarily in epithelial tissues. GSDMD is activated by caspases in response to inflammasome activators where it drives pyroptosis during microbial infection and danger signals. Down regulation of GSDMD is found in gastric cancer and mutations in GSDMD have been found in a variety of cancers.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human GSDMD Capture Antibody 1 x 600µl 10X Human GSDMD Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human GSDMD Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Sequence similarities
Belongs to the gasdermin family. - Information by UniProt
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Alternative names
- 1810036L03Rik
- DF 5L
- DF5L
see all -
Database links
- Entrez Gene: 79792 Human
- Omim: 617042 Human
- SwissProt: P57764 Human
- Unigene: 118983 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human GSDMD standard curve in 1X Cell Extraction Buffer PTR.The GSDMD standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native GSDMD in human A549, Jurkat and liver homogenate extracts based on 1,000 µg/mL, 250 µg/mL, and 1,000 µg/mL extract loads, respectively.The concentrations of GSDMD were measured in duplicate and interpolated from the GSDMD standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GSDMD concentration was determined to be 25.76 ng/mL in A549, 30.24 ng/ml in Jurkat, and 9.19 ng/ml in liver homogenate extract.
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Sandwich ELISA - Human GSDMD ELISA Kit (ab272463)To learn more about the advantages of recombinant antibodies see here.
