Anti-COLEC11 antibody [EPR24192-169] (ab278081)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24192-169] to COLEC11
- Suitable for: IP, WB
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-COLEC11 antibody [EPR24192-169]
See all COLEC11 primary antibodies -
Description
Rabbit monoclonal [EPR24192-169] to COLEC11 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB MouseHuman -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human plasma; Mouse plasma; Human kisney, adrenal gland, liver, skeletal muscle and stomach tissue lysates. IP: Mouse plasma.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24192-169 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-COLEC11 antibody [EPR24192-169] (ab278081) at 1/1000 dilution
Lane 1 : Human kidney tissue lysate
Lane 2 : Human adrenal gland tissue lysate
Lane 3 : Human liver tissue lysate
Lane 4 : Human skeletal muscle tissue lysate
Lane 5 : Human stomach tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 28,34 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID:30323815, 28772263, 20956340).
Low expression: skeletal muscle, stomach (PMID:20956340).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 81 seconds.
-
COLEC11 was immunoprecipitated from 0.35 mg Mouse plasma with ab278081 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab278081 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse plasma 10 μl
Lane 2: ab278081 IP in Mouse plasma
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab278081 in Mouse plasma
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
-
All lanes : Anti-COLEC11 antibody [EPR24192-169] (ab278081) at 1/1000 dilution
Lane 1 : Human plasma
Lane 2 : Mouse plasma
Lysates/proteins at 20 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 28,34 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:28772263, 30323815).
Exposure time: 3 minutes.
-