Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human GLP1 (7-36) ELISA Kit  (ab184857)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 25 pg/ml
  • Range: 59.6 pg/ml - 1000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human GLP1 (7-36) ELISA Kit 
    See all GLP1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Human serum 8 7%
    Inter-assay
    Sample n Mean SD CV%
    Human serum 3 9%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    25 pg/ml

  • Range

    59.6 pg/ml - 1000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 109 105% - 112%
    Cell culture media 99 93% - 102%
    Hep Plasma 110 104% - 112%
    Cit plasma 115 107% - 123%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Sheep, Horse

  • Product overview

    Human GLP1 (7-36) ELISA kit (ab184857) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GLP-1(7-36) active peptide protein in human serum, plasma and culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate human GLP1 with 25 pg/mL sensitivity.

    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips

    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.


    ASSAY SPECIFICITY


    GLP-1(9-36) recombinant protein was diluted at 10ng/mL and assayed for cross reactivity. Signal was found at 7% from GLP-1 (7-36) protein assayed at the same concentration.


    SPECIES REACTIVITY


    This kit recognizes both native and recombinant human GLP-1 protein in the serum and plasma samples.


    Due to sequence similarity, this kit is predicted to cross react with multiples species such as mouse, rat, bovine, sheep, rabbit, pig and horse.  

  • Notes

    GLP-1 (Glucagon like peptide 1) is part of the group of incretin hormones that are secreted by the gastrointestinal tract in response to food intake to assist glucose stimulated insulin secretion and glucagon suppression. GLP-1 is a 30 aminoacid peptide cleaved from proglucagon and released by the L-cells of the distal ileum. The intracellular precursor of GLP-1 (1-37) is cleaved to form the active peptides GLP-1 (7-37) and GLP-1 (7-36)NH2. The active peptides bind to the GLP-1 receptor (GLP-1r) expressed in the pancreatic beta cell and are quickly metabolized by the enzyme dipeptidyl peptidase IV (DPP-IV) to form the peptide GLP-1 (9-36), which has no insulin stimulating activity. Binding of active GLP-1 to the receptor, increases cAMP levels and potentiates insulin secretion via Protein Kinase A (PKA) and the cAMP-regulated nucleotide exchange factor (Epac2).
    GLP-1 and its receptor are also suggested to play a role in the central nervous systems as mediators of satiety. Intracerebroventricular GLP-1 has been shown to induce c-FOS activity in the hypothalamus and the central nucleus of the amygdala, both of which are important in the regulation of appetite.

     

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human GLP1 (7-36) Capture Antibody 1 x 600µl
    10X Human GLP1 (7-36) Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human GLP1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Relevance

    Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.

  • Cellular localization

    Secreted.

  • Alternative names

    • GCG
    • Glicentin
    • Glicentin-related polypeptide
    • GLP-1(7-36)
    • GLP-1(7-37)
    • GLP1
    • GLP2
    • Glucagon
    • Glucagon-like peptide 1
    • Glucagon-like peptide 1(7-36)
    • Glucagon-like peptide 1(7-37)
    • Glucagon-like peptide 2
    • GRPP
    • Incretin hormone
    • OXM
    • OXY
    • Oxyntomodulin
    see all

  • Database links

    Images

    • Other - Human GLP1 (7-36) ELISA Kit  (ab184857)
      Other - Human GLP1 (7-36) ELISA Kit  (ab184857)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of GLP-1 standard curve.
      Example of GLP-1 standard curve.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Standard curve
      Standard curve

      Example of GLP-1 standard curve.  The GLP-1 standard curve was prepared as described.  Raw data values are shown in the table.  Background-subtracted data values (mean +/- SD) are graphed.

    • Ten individual healthy donors were evaluated for the presence of GLP-1 in serum using this assay.
      Ten individual healthy donors were evaluated for the presence of GLP-1 in serum using this assay.

      Serum of each donor was diluted 1:10 using sample diluent NS. The mean levels of GLP-1, after adjusting for dilution factor, were found at 433.5 pg/mL with a standard deviation of 277 pg/mL.

       

    • Sample Data
      Sample Data

      Ten individual healthy donors were evaluated for the presence of GLP-1 in serum using this assay. Serum of each donor was diluted 1/10 using sample diluent NS. The mean levels of GLP-1, after adjusting for dilution factor, were found at 433.5 pg/mL with a standard deviation of 277 pg/mL.

    • Linearity of dilution.
      Linearity of dilution.

      Recombinant GLP-1 (7-36) was spiked into the following biological samples and diluted in  a 2-fold dilution series in Sample Diluent NS. 

      Linearity of dilution is determined based on interpolated values from the standard curve.  Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

    • Sandwich ELISA - Human GLP1 (7-36) ELISA Kit  (ab184857)
      Sandwich ELISA - Human GLP1 (7-36) ELISA Kit  (ab184857)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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