Human GCP-2 ELISA Kit (CXCL6) (ab260055)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 13.1 pg/ml
- Range: 25 pg/ml - 1600 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human GCP-2 ELISA Kit (CXCL6)
See all GCP2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.1% Inter-assay Sample n Mean SD CV% Serum 3 2.4% -
Sample type
Cell culture supernatant, Serum, Cell culture media, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
13.1 pg/ml -
Range
25 pg/ml - 1600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 94 90% - 101% Serum 86 83% - 88% Cell culture media 99 93% - 105% EDTA Plasma 81 78% - 85% Cit plasma 95 94% - 96% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human GCP-2 ELISA Kit (CXCL6) (ab260055) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GCP-2 (CXCL6) protein in cit plasma, edta plasma, serum, cell culture media, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GCP-2 (CXCL6) with 13.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
GCP-2 (Granulocyte chemotactic protein 2), also known as CXCL6 (C-X-C motif chemokine 6) is a chemotactic factor for neutrophil granulocytes. GCP-2 signals through binding and activation of its receptors CXCR1 and CXCR2. In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human GCP-2 (CXCL6) Capture Antibody 1 x 600µl 10X Human GCP-2 (CXCL6) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human GCP-2 (CXCL6) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Chemotactic for neutrophil granulocytes. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C X C motif chemokine 6
- Chemokine (C X C motif) ligand 6
- Chemokine alpha 3
see all -
Database links
- Entrez Gene: 6372 Human
- Omim: 138965 Human
- SwissProt: P80162 Human
- Unigene: 164021 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human GCP-2 standard curve comparisonStandard Curve comparison between human GCP-2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human GCP-2 standard curve in Sample Diluent NS.The GCP-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native GCP-2 in human PBMC cell culture supernatants derived from cells seeded at 107 cells/mL and cultured in the presence or absence of 1.5% PHA for 36 hours.The concentrations of GCP-2 were measured in duplicates, interpolated from the GCP-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC cell culture supernatant (stimulated) 50% and PBMC cell culture supernatant (unstimulated) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GCP-2 concentration was determined to be 1,064 pg/mL in neat stimulated PBMC cell culture supernatant and undetectable in unstimulated PBMC cell culture supernatant.
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Sandwich ELISA - Human GCP-2 ELISA Kit (CXCL6) (ab260055)To learn more about the advantages of recombinant antibodies see here.
