Human FMRP ELISA KIT (ab224885)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 99.67 pg/ml
- Range: 390.63 pg/ml - 25000 pg/ml
- Sample type: Cell culture extracts, Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human FMRP ELISA KIT
See all FMRP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 3.6% Inter-assay Sample n Mean SD CV% Extract 3 4.4% -
Sample type
Cell culture extracts, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
99.67 pg/ml -
Range
390.63 pg/ml - 25000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 93 90% - 97% Cell Lysate 108 104% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human FMRP ELISA KIT (ab224885) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FMRP protein in cell lysate and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FMRP with 99.67 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human FMRP is a 632 amino acid protein coded by the FMR1 gene. It is expressed in neurons, brain, testis, placenta and lymphocytes, epithelial tissues and at very low levels in glial cells. The most reported role for FMRP is of a regulator of translation. It is a component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression RNA-binding protein activity in intracellular RNA transport and in the regulation of translation of target mRNAs. Reports suggest it is associated with polysomes and may play a role in the transport of mRNA from the nucleus to the cytoplasm.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human FMRP Capture Antibody 1 x 600µl 10X Human FMRP Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human FMRP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C). -
Tissue specificity
Highest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells. -
Involvement in disease
Defects in FMR1 are the cause of fragile X syndrome (FRAX) [MIM:300624]. Fragile X syndrome is a common genetic disease (has a prevalence of one in every 2000 children) which is characterized by moderate to severe mental retardation, macroorchidism (enlargement of the testicles), large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
Defects in FMR1 are the cause of fragile X tremor/ataxia syndrome (FXTAS) [MIM:300623]. In FXTAS, the expanded repeats range in size from 55 to 200 repeats and are referred to as 'premutations'. Full repeat expansions with greater than 200 repeats results in fragile X mental retardation syndrome [MIM:300624]. Carriers of the premutation typically do not show the full fragile X syndrome phenotype, but comprise a subgroup that may have some physical features of fragile X syndrome or mild cognitive and emotional problems.
Defects in FMR1 are the cause of premature ovarian failure syndrome type 1 (POF1) [MIM:311360]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. -
Sequence similarities
Belongs to the FMR1 family.
Contains 2 KH domains. -
Post-translational
modificationsPhosphorylated on several serine residues. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. - Information by UniProt
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Alternative names
- FMR 1
- Fmr1
- Fmr1 gene
see all -
Database links
- Entrez Gene: 2332 Human
- Omim: 309550 Human
- SwissProt: Q06787 Human
- Unigene: 103183 Human
Images
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SimpleStep ELISA Protocol Diagram
The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
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Example of human FMRP standard curve in 1X Cell Extraction Buffer PTR.The FMRP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native FMRP in SH-SY5Y cell extract and Jurkat cell extract based on a 1,000 µg/mL and 500 µg/mL extract load, respectively.The concentrations of FMRP were measured in duplicate and interpolated from the FMRP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FMRP concentration was determined to be 8,545 pg/mL in SH-SY5Y cell extract and 6,733 pg/mL in Jurkat cell extract.
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Sandwich ELISA - Human FMRP ELISA KIT (ab224885)To learn more about the advantages of recombinant antibodies see here.
