Human Fibromodulin ELISA Kit (ab275895)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.08 ng/ml
- Range: 0.19 ng/ml - 12 ng/ml
- Sample type: Cell culture media, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Fibromodulin ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 5.2% Inter-assay Sample n Mean SD CV% Serum 4 5.9% -
Sample type
Serum, Cell Lysate, Cell culture media, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.08 ng/ml -
Range
0.19 ng/ml - 12 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 114 111% - 117% Cell culture media 110 105% - 116% Hep Plasma 103 100% - 105% EDTA Plasma 103 102% - 105% Cit plasma 114 111% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Fibromodulin ELISA kit (ab275895) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Fibromodulin protein in human serum, plasmas, cell culture supernatant, and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Fibromodulin with 0.08 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Fibromodulin (FMOD), also referred to as Collagen-binding 59 kDa protein and Keratan sulfate proteoglycan fibromodulin, is phosphoprotein encoded by the FMOD gene. FMOD belongs to the small leucine-rich proteoglycan class II family. FMOD has up to four N-linked keratan sulfate chains within the protein’s leucine-rich region FMOD binds collagen type I and II fibrils and may be involved in the assembly of extracellular matrix, as well as possibly sequestering TGF-beta into the extracellular matrix.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Fibromodulin Capture Antibody 1 x 600µl 10X Human Fibromodulin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human Fibromodulin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Affects the rate of fibrils formation. May have a primary role in collagen fibrillogenesis. -
Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
Post-translational
modificationsBinds keratan sulfate chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- Collagen binding 59 kDa protein
- Collagen-binding 59 kDa protein
- Fibromodulin
see all -
Database links
- Entrez Gene: 2331 Human
- Omim: 600245 Human
- SwissProt: Q06828 Human
- Unigene: 519168 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Fibromodulin standard curve in Sample Diluent NS.The Fibromodulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Fibromodulin in human serum, plasma (EDTA), plasma (citrate), plasma (heparin).The concentrations of Fibromodulin were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (EDTA) 25%, plasma (citrate) 50%, plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 21.9 ng/mL in serum, 22.5 ng/mL in plasma (EDTA) and 18.3 ng/mL in and plasma (citrate), and 20.2 ng/mL in plasma (heparin).
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Interpolated concentrations of native Fibromodulin in human colon on a 300 µg/mL extract load.The concentrations of Fibromodulin were measured in duplicate and interpolated from the Fibromodulin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Serum from eight individual healthy Fibromodulin female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibromodulin concentration was determined to be 31.9 ng/mL with a range of 22.5 – 55.0 ng/mL.
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Sandwich ELISA - Human Fibromodulin ELISA Kit (ab275895)To learn more about the advantages of recombinant antibodies see here.