Human Fatty Acid Oxidation In-Cell ELISA Kit (ab118182)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Colorimetric
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Human Fatty Acid Oxidation In-Cell ELISA Kit
See all Fatty Acid Oxidation kits -
Detection method
Colorimetric -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
ab118182 uses quantitative immunocytochemistry to measure protein levels or post-translational modifications in cultured cells. Cells are fixed in a microplate and targets of interest are detected with highly specific, well-characterized monoclonal antibodies, and levels are quantified with IRDye®-labeled Secondary Antibodies. IR imaging and quantitation is performed using a LI-COR® Odyssey® or Aerius® system.
Plates are available in our ICE (In-Cell ELISA) Support Pack (ab111542) which can be bought seperately.
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Notes
Upon receipt spin down the contents of the IRDye®-labeled Secondary Antibody tube and protect from light. Store all components upright at 4C. This kit is stable for at least 6 months from receipt.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 1000X IRDye 800-labeled Secondary antibody 1 x 0.04ml 100X ACADM Primary Antibody 1 x 0.12ml 100X ACADVL Primary Antibody 1 x 0.12ml 100X HADHA Primary Antibody 1 x 0.12ml 10X Blocking Buffer 1 x 20ml 10X Phosphate Buffered Saline 1 x 100ml 33x Triton X-100 1 x 5ml 400X Tween-20 1 x 4ml 1X Janus Green Stain 1 x 17ml -
Research areas
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Alternative names
- Beta Oxidation
- Beta-Oxidation
Images
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In-Cell ELISA - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)Panel A shows levels of ACADVL protein in a deficient cell line (ACADVL:p.[N122D]).
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In-Cell ELISA - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)Panel C shows levels of HADHA protein in two deficient cell lines: (1) HADHA:p.[E474Q] and (2) HADHB:p. [R61H];[R247H]. Note that although LCHAD#1 deficient has a partial deficiency as observed by ICC. -
In-Cell ELISA - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)Panel B shows levels of ACADM protein in two deficient cell lines (ACADM:p. [K304E]). -
Immunocytochemistry - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)Antibody specificity demonstrated by immunocytochemistry. Visualization under the microscope was carried out with a completely opened aperture and a very narrow field of visualization at 40x. Panel A shows control fibroblasts and panel B shows deficient fibroblasts. Left panel shows staining with anti-ACADVL ab, center panel with anti-ACADM ab and right panel with anti-HADHA ab. Note that although LCHAD#1 deficient had a characterized homozygous mutation, the antibody shows a mosaic pattern of staining with about 1/3 of the cells lacking HADHA staining and an overall 40% reduction of signal as observed by ICE.